14 December 1995 AerosprayŽ Cytology Slide Stainer SS-151 Papanicolaou Reagent Concentrate System Intended Use The SS-151 system is a series of concentrated Papanicolaou reagents for staining cytological specimens with the Wescor Aerospray Cytology Slide Stainer. Description Wescor reagents provide optimum performance with your Aerospray slide stainer. The reagent concentrate system delivers the quality and performance of our standard prepared reagents in a smaller package. Reagent concentrates offer lower cost, less shipping expense and reduced storage space requirements. You can use diluted reagent concentrates immediately after mixing. For convenient mixing and storage, we recommend a five liter space-saver container and dispensing spigot for each reagent. These reusable items are available from Wescor: Catalog No. Description AC-038 Five Liter Space-Saver Container with Lid AC-039 Dispensing Spigot for Space-Saver Container AC-043 Empty 500 mL Reagent Bottle with Lid Papanicolaou Reagent Concentrates Catalog No. Description SS-151A EA-50 Concentrate (Part A), 205 mL will make 4.5 liters intermediate solution. EA-50 Concentrate (Part B), when mixed with diluted SS-151A Part A and 13 mL acetic acid will make 4.5 liters Reagent A, EA-50 Stain. SS-151B Orange G Concentrate, 205 mL will make 4.5 liters Reagent B, Orange G Stain. SS-151C Bluing Agent Concentrate, 205 mL will make 4.5 liters Reagent C, Bluing Agent. SS-151D Hematoxylin I Concentrate, 500 mL will make 5.0 liters Reagent D, Hematoxylin I. SS-151D2 Hematoxylin II Concentrate, 1000 mL will make 5.0 liters Reagent D, Hematoxylin II. N/A Alcohol Wash (equivalent to SS-051E) can be prepared from customer's supplies (see table). Each reagent concentrate includes: Reagent Concentrate Reagent Label for Five Liter Space-Saver Container Reagent Label for 500 mL Reagent Bottle Mixing Instructions (One per Order) Instructions WARNING! These reagents contain moderately toxic chemicals that require care in handling. Always use appropriate safety measures including gloves and eye protection when handling reagents. Refer to the Material Safety Data Sheet (MSDS) for more information. PREPARE CONTAINER Use a clean, empty container with five liter capacity. If you notice any debris or precipitated reagent from previous use, clean the container before proceeding. ADD SOLVENTS and CONCENTRATES Add solvents and concentrates to the container in the order and quantity listed below (reserve a small amount of appropriate solvent to rinse each concentrate bottle, ensuring that all concentrated reagent is diluted): REAGENT WATER1 ETHANOL2 CONCENTRATE OTHER A 700 mL 3,600 mL SS-151A (Part A) 205 mL SS-151A (Part B) - 14 mL Acetic Acid - 13 mL B 335 mL 3,960 mL SS-151B 205 mL NONE C 3,850 mL 450 mL SS-151C 205 mL NONE D (Hematoxylin I) 3,375 mL NONE SS-151D3 500 mL Ethylene Glycol 1,125 mL D (Hematoxylin II) 3,000 mL NONE SS-151D23 1,000 mL Ethylene Glycol 1,000 mL E NONE 2,500 mL NONE Isopropanol4 2,500 mL 1 Filtered, Deionized Water (0.2 micron filter, minimum 200,000 ohms resistivity) 2 Anhydrous, Reagent Grade SD3A Ethanol 3 This solution will solidify at temperatures below 4° C (40° F). Should this occur in shipping or storage, place the concentrate bottle in a beaker of very hot water (70 - 100° C) for ten minutes then shake until completely dissolved. 4 Anhydrous, Reagent Grade Isopropanol (analyzed water content less than 0.05%) MIX Replace the dispensing spigot or lid and mix the reagent thoroughly by inverting the container several times. APPLY LABELS Transfer the lot and expiration data from the concentrate bottle to both reagent labels. Initial and date the reagent labels. Apply the appropriate label to both the mixing container and the instrument reagent bottle. The reagent is ready for use. Note: Before adding diluted reagent to the instrument bottles, check each bottle for debris or precipitated reagent. If necessary, clean the instrument bottle before refilling. Alternative Instructions to Prepare 500 mL of Reagent You may find it more convenient to prepare only 500 mL of reagent at a time, eliminating the need to store a large volume of prepared reagent. WARNING! These reagents contain moderately toxic chemicals that require care in handling. Always use appropriate safety measures including gloves and eye protection when handling reagents. Refer to the Material Safety Data Sheet (MSDS) for more information. PREPARE CONTAINER Use a clean, empty reagent bottle (500 mL capacity). If you notice any debris or precipitated reagent from previous use, clean the bottle before proceeding. ADD SOLVENTS and CONCENTRATES Add solvents and concentrates to the container in the order and quantity listed below (reserve a small amount of appropriate solvent to rinse each concentrate bottle, ensuring that all concentrated reagent is diluted): REAGENT WATER1 ETHANOL2 CONCENTRATE OTHER A 78 mL 400 mL SS-151A (Part A) 23 mL SS-151A (Part B) - 1.6 mL Acetic Acid - 1.4 mL B 37 mL 440 mL SS-151B 23 mL NONE C 428 mL 50 mL SS-151C 23 mL NONE D (Hematoxylin I) 338 mL NONE SS-151D3 50 mL Ethylene Glycol 113 mL D (Hematoxylin II) 300 mL NONE SS-151D23 100 mL Ethylene Glycol 100 mL E NONE 250 mL NONE Isopropanol4 250 mL 1 Filtered, Deionized Water (0.2 micron filter, minimum 200,000 ohms resistivity) 2 Anhydrous, Reagent Grade SD3A Ethanol 3 This solution will solidify at temperatures below 4° C (40° F). Should this occur in shipping or storage, place the concentrate bottle in a beaker of very hot water (70 - 100° C) for ten minutes then shake until completely dissolved. 4 Anhydrous, Reagent Grade Isopropanol (analyzed water content less than 0.05%) MIX Replace the lid and mix the reagent thoroughly by inverting the bottle several times. APPLY LABEL Transfer the lot and expiration data from the concentrate bottle to the reagent label. Initial and date the reagent label. The reagent is ready for use.